Farideh Saberi, Omar Youssef, Arto Kokkola+4 more · 2024 · Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences · added 2026-04-24
Stools from colorectal cancer patients are noninvasive samples that could be used to compare the frequency of hotspot mutations between two different ethnic cohorts. We collected stool samples from th Show more
Stools from colorectal cancer patients are noninvasive samples that could be used to compare the frequency of hotspot mutations between two different ethnic cohorts. We collected stool samples from the Iranian cohort (52 patients and 49 controls) and the Finnish cohort (40 patients and 14 controls). Following stool DNA extraction, we used the AmpliSeq Colon and Lung Cancer panel to prepare DNA libraries before sequencing. The Iranian cohort exhibited 35 hotspot mutations in the Genes involved in MAPK and PI3K-MAPK pathways showed a higher frequency of mutations in Iranian patients which may have therapeutic implications. Show less