👤 Mehmet Kose

Hepatitis B is a significant public health issue worldwide. Long noncoding RNAs (lncRNAs) are pivotal in biological mechanisms. The involvement of lncRNAs in hepatitis Hepatitis B remains incompletely understood. This study sought to explore the potential of certain lncRNAs as diagnostic and prognostic markers in hepatitis B, and their ability to differentiate between clinical disease subgroups. Plasma samples from 204 individuals diagnosed with hepatitis B were meticulously examined for the presence of specific lncRNAs, focusing on those known to be associated with hepatitis B as indicated in the literature. In this study, gene expression levels of 14 lncRNAs were analyzed in three subgroups (chronic, recovered and inactive HBsAg carriers) and compared with those in the healthy control group. The downregulation of the RN7SL1 and Alpha-280 genes in all patients indicates potential diagnostic lncRNAs. ZFHX2-AS1 expression was consistently lower in all subgroups, while SRA1 showed high expression in recovered hepatitis B patients, indicating that it is a descriptive marker. DLG2-AS2 expression increased in the chronic hepatitis B but decreased in the other groups. These findings reveal the prognostic importance of lncRNAs in hepatitis B. The expression levels of ZFHX2-AS1, RN7SL1, PCAT-1, PCA3, SRA1 and Alpha-280 have been identified as crucial biomarkers for the diagnosis of hepatitis B and for distinguishing between its clinical subgroups. A strong association between hepatitis B disease and the gene expression of the DLG2-AS2, HOTTIP, HOTAIRM1, HOXA11-AS, NOS2P3, LINC02665, MEG9, and RNY5 lncRNAs has been suggested. Show less

The aim was to evaluate the expression of genes of steroidogenic, cholesterol uptake, and liver X receptor (LXR) mediated cholesterol efflux pathway in ovine corpus luteum (CL) during natural and prostaglandin F2α (PGF2α) induced luteolysis and early pregnancy. For this study, two experiments were carried out 1); ewes were grouped into two sub-groups as cyclic 12 (C12, n = 4) and 16 (C16, n = 4) and pregnant 12 (P12, n = 4), 16 (P16, n = 4), and 22 (P22, n = 4). Additionally, 2) ewes were grouped into four groups following treatment of PGF2α, the duration of PGF2α challenge at 1 (PG1, n = 4), 4 (PG4, n = 4), and 16 (PG16, n = 4) hours on day 12 of the cycle was compared with 0 h. The corpus luteum tissue samples were collected on the corresponding estrus cycle and pregnancy days, and RNA was extracted using Trizol. mRNA expression levels of the steroidogenic (StAR, CYP11A1, and HSD3B1) and cholesterol uptake receptors (SCARB1 and LDLR) and LXR pathway (NR1H3, NR1H2, ABCA1, and ABCG1) were assessed using quantitative PCR (qPCR), and protein of LXR pathway was investigated using western blot. In-situ hybridization was used to detect mRNA localization. Steroidogenic and cholesterol uptake mRNAs were decreased in C16, while NR1H2 and ABCG1 were increased in C16, compared to C12. Steroidogenic and cholesterol uptake mRNA was greater in P16 than in C16. NR1H2 and ABCA1 protein expression were higher in P16 than in C16. LDLR mRNA was higher in P22 than in P12, while SCARB1 was higher in P16 than in P12. NR1H2 mRNA was greater in P22 than in P12. Steroidogenic and cholesterol uptake mRNA were decreased in PGF2α-induced luteolysis groups against C12. ABCG1 mRNA was higher in PG16 than in PG4 and PG1. The reduction of lipoprotein receptors rather than LXR-mediated reverse transport may contribute to the decline in progesterone (P4) in natural and functional luteolysis. Show less