👤 Seyed Morteza Tayebi

Obesity is a significant public health issue associated with an elevated risk of chronic diseases. Discovering appropriate exercise interventions combined with beneficial herbal supplements has always been investigated. Hence, our study aimed to assess the impact of 12 weeks of high-intensity interval training (HIIT) and spirulina supplementation on the apolipoproteins, insulin resistance, and body composition of men with obesity. Forty-four men with obesity (height: 168.42 ± 2.63 cm, body mass: 93.24 ± 2.23 kg; BMI: 32.89 ± 1.23 kg/m Show less

Induced pluripotent stem cells (iPSC) derived from healthy individuals are important controls for disease-modeling studies. Here we apply precision health to create a high-quality resource of control iPSCs. Footprint-free lines were reprogrammed from four volunteers of the Personal Genome Project Canada (PGPC). Multilineage-directed differentiation efficiently produced functional cortical neurons, cardiomyocytes and hepatocytes. Pilot users demonstrated versatility by generating kidney organoids, T lymphocytes, and sensory neurons. A frameshift knockout was introduced into MYBPC3 and these cardiomyocytes exhibited the expected hypertrophic phenotype. Whole-genome sequencing-based annotation of PGPC lines revealed on average 20 coding variants. Importantly, nearly all annotated PGPC and HipSci lines harbored at least one pre-existing or acquired variant with cardiac, neurological, or other disease associations. Overall, PGPC lines were efficiently differentiated by multiple users into cells from six tissues for disease modeling, and variant-preferred healthy control lines were identified for specific disease settings. Show less