👤 Ahmed A Elolimy

The current study evaluated the impact of lysophospholipid emulsifiers' (LPLs) dietary incorporation on ameliorating the negative impacts of oxidative stress in broilers. A total of 270 2-week-old male Avian 48 chicks were randomly divided into six experimental groups. The first group fed a basal diet (BD) only, while the second group (+DEX) received BD containing 2 mg/kg dexamethasone. The third and fourth groups consisted of birds fed a BD containing 0.5 and 1 g of LPLSs/kg, respectively. The fifth and sixth groups, received BD containing 1 mg/kg dexamethasone and were supplemented with 0.5 and 1 g of LPLs, respectively. Separate supplementation of LPLs significantly improved the broilers' growth as confirmed by increasing final weight, body gain, and FI with improved feed conversion ratio (FCR) ( Dietary supplementation of LPLs at 0.5g level could effectively mitigate the adverse effects of oxidative stress in broilers, improving the growth performance, immune response, intestinal health, and meat quality of broiler chickens under normal and stressful conditions. Show less

Although choline requirements are unknown, enhanced postruminal supply may decrease liver triacylglycerol (TAG) storage and increase flux through the methionine cycle, helping cows during a negative energy balance (NEB). The objective was to investigate effects of postruminal choline supply during NEB on hepatic activity of betaine-homocysteine methyltransferase (BHMT), methionine synthase (MTR), methionine adenosyltransferase, transcription of enzymes, and metabolite concentrations in the methionine cycle. Ten primiparous rumen-cannulated Holstein cows (158 ± 24 d postpartum) were used in a replicated 5 × 5 Latin square design with 4-d treatment periods and 10 d of recovery (14 d/period). Treatments were unrestricted intake with abomasal infusion of water (A0), restricted intake (R; 60% of net energy for lactation requirements to induce NEB) with abomasal infusion of water (R0) or R plus abomasal infusion of 6.25, 12.5, or 25 g/d of choline ion. Liver tissue was collected on d 5 after the infusions ended, blood on d 1 to 5, and milk on d 1 to 4. Statistical contrasts were A0 versus R0 (CONT1) and tests of linear (L), quadratic (Q), and cubic (C) effects of choline dose. Plasma choline increased with R (CONT1) and choline (L). Although R decreased milk yield (CONT1), choline increased milk yield and liver phosphatidylcholine (PC), but decreased TAG (L). No differences were observed in plasma PC or very-low-density lipoprotein concentrations with R or choline. Activity and mRNA abundance of BHMT were greater with R (CONT1) and increased with choline (L). Although activity of MTR was lower with R (CONT1), it tended to increase with choline (L). No effect of R was detected for activity of methionine adenosyltransferase, but it changed cubically across dose of choline. Those responses were associated with linear increases in the concentrations of liver tissue (+13%) and plasma methionine concentrations. The mRNA abundance of CPT1A, SLC22A5, APOA5, and APOB, genes associated with fatty acid oxidation and lipoprotein metabolism, was upregulated by choline (Q). Overall, enhanced supply of choline during NEB increases hepatic activity of BHMT and MTR to regenerate methionine and PC, partly to help clear TAG. The relevance of these effects during the periparturient period merits further research. Show less