A prior study in bovine mammary (MACT) cells indicated that long-chain fatty acids (LCFA) C16:0 and C18:0, but not unsaturated LCFA, control transcription of milk fat-related genes partly via the acti Show more
A prior study in bovine mammary (MACT) cells indicated that long-chain fatty acids (LCFA) C16:0 and C18:0, but not unsaturated LCFA, control transcription of milk fat-related genes partly via the activation of peroxisome proliferator-activated receptor gamma (PPARĪ³). However, in that study, the activation of PPARĪ³ by LCFA was not demonstrated but only inferred. Prior data support a lower response of PPARĪ³ to agonists in goat mammary cells compared to bovine mammary cells. The present study aimed to examine the hypothesis that LCFA alter the mRNA abundance of lipogenic genes in goat mammary epithelial cells (GMEC) at least in part via PPARĪ³. Triplicate cultures of GMEC were treated with a PPARĪ³ agonist (rosiglitazone), a PPARĪ³ inhibitor (GW9662), several LCFA (C16:0, C18:0, Show less