👤 Gurler Akpinar

Prostate cancer is the second leading cause of cancer-related deaths in men. Periodontitis is considered a high-risk factor for prostate cancer, but the genetic mechanism is unclear. This study aims to identify dysregulated miRNAs, their associated genes, signaling pathways, and compounds linking periodontitis to prostate cancer. The miRNA expression datasets of prostate cancer and periodontitis were obtained from the GEO database. Differentially expressed miRNAs (DEmiRNAs) were identified, and common DEmiRNAs (Co-DEmiRNAs) between both datasets were determined. The Co-DEmiRNA-target network structure and functional analyses, including miRNet 2.0, were performed, encompassing Co-DEmiRNA-gene, Co-DEmiRNA-transcription factor (TF), and Co-DEmiRNA-compound networks. Functional enrichment analysis for Co-DEmiRNA genes and Co-DEmiRNA-TF networks was conducted using KEGG, Reactome pathways, and Gene Ontology (GO). Co-up and co-down DEmiRNAs were validated with TCGA miRNA-seq data. hsa-mir-148a-3p, hsa-mir-148b-5p, and hsa-mir-623 are the top miRNA nodes in Co-DEmiRNA-Target networks. The most significant candidate miRNA dysregulation genes are POU2F1, TMOD3, SCD, PRRC2C, and MAT2A, while the most important dysregulation TF includes TP53, CREB1, DNMT1, E2F1, and EGR1. Arsenic trioxide, gemcitabine, and 1,2,6-tri-O-galloyl-beta-D-glucopyranose are the most correlated compounds. Functional analyses revealed multiple cell signaling pathways, such as NOTCH and CREB phosphorylation, and regulation of processes, such as RNA metabolism and transcription. Our study suggests candidate molecular mechanisms linking periodontitis to prostate cancer, highlighting potential compounds targeting both diseases. These findings provide a foundation for guiding future basic and clinical research. Show less

The goal of this study was to pinpoint potential molecular pathways that may have contributed to the onset of pseudoexfoliation syndrome (PEX), a systemic illness associated with aging that has no known cause and is brought on by the deposition of fibrillary white flaky debris in ocular tissues. Protein pools representing each group were created using two-dimensional gel electrophoresis (2DE) in conjunction with a matrix-assisted laser desorption ionization-time of flight/time of flight (MALDI-TOF/TOF) mass spectrometer. Aqueous humor (AH) from patients with PEX and cataracts was also collected for a comprehensive study of the data; ingenuity pathway analysis (IPA) was used for the discovered proteins. In comparison to controls, 2DE showed that 10 sites in PEX patients had differently altered gene expression. Two of these proteins, transthyretin (TTR) and apolipoprotein A4 (ApoA4) were significantly overexpressed in PEX patients, but the remaining proteins were only mildly altered. The liver X receptor (LXR) and the retinoid X receptors (RXR) may play a crucial role in the pathophysiology of PEX according to IPA employing these 10 proteins. The altered proteins, particularly ApoA4 and TTR, may be important in revealing the molecular process behind PEX, as anticipated by IPA. Toprak M, Yuksel N, Akpinar G, Show less