👤 Andrei Vacaru

While social robots show promise for therapeutic interventions, accurate assessments of (vulnerable) participants' affective outcomes require attention. The careful selection of devices for recording autonomic processes in response to stress-inducing and relaxing exercises is essential to ensure data quality recordings and participants' comfort. This foundational study assessed two commonly utilized devices to record electrodermal activity (EDA), indexed through skin conductance, concerning their sensitivity to stress-relaxation manipulations and social validity during a robot intervention: a sock with a Shimmer device and a wrist-worn Empatica E4. We aimed to select the most sensitive and easy-to-wear one as a precursor to a larger intervention study featuring mindfulness-based relaxation exercises delivered by an NAO robot. The findings, based on 28 healthy Dutch-speaking adult volunteers wearing both devices, revealed sensitivity in detecting EDA variations in arousal following stressful (increase) and Robot-delivered mindfulness-based relaxation (decrease) exercises, further corroborated by self-reports. Bland-Altman results suggested little agreement between the two devices and lower sensitivity for the Empatica E4. No statistically significant differences concerning wearing comfort between the Empatica E4 and the Shimmer devices emerged. Although both devices independently showed sensitivity to stress/relaxation manipulation, the choice for one or the other should be informed by the activities in the intervention. Show less

Dep1 is a transmembrane protein-tyrosine phosphatase (PTP) that is expressed in vascular endothelial cells and has tumor suppressor activity. Mouse models with gene targeted Dep1 either show vascular defects, or do not show any defects at all. We used the zebrafish to investigate the role of Dep1 in early development. The zebrafish genome encodes two highly homologous Dep1 genes, Dep1a and Dep1b. Morpholinos specific for Dep1a and Dep1b induced defects in vasculature, resulting in defective blood circulation. However, Green Fluorescent Protein expression in fli1a::gfp1 transgenic embryos and cdh5 expression, markers of vascular endothelial cells, were normal upon Dep1a- and Dep1b-MO injection. Molecular markers indicated that arterial specification was reduced and venous markers were expanded in Dep1 morphants. Moreover, the Dep1a/Dep1b knockdowns were rescued by inhibition of Phosphatidylinositol-3 kinase (PI3K) and by expression of active Notch and Grl/Hey2. Our results suggest a model in which Dep1 acts upstream in a signaling pathway inhibiting PI3K, resulting in expression of Notch and Grl, thus regulating arterial specification in development. Show less