Perfluorooctanesulfonic acid (PFOS) is a persistent environmental pollutant widely present in ecosystems and humans, linked to numerous diseases, including cancers, due to its potent toxicity. Despite Show more
Perfluorooctanesulfonic acid (PFOS) is a persistent environmental pollutant widely present in ecosystems and humans, linked to numerous diseases, including cancers, due to its potent toxicity. Despite its harmful effects, effective strategies for mitigating PFOS-induced toxicity remain undeveloped or at best underdeveloped. In this effort, we explore the concept of toxicity reversal by loci-specific DNA methylation editing to reverse PFOS-induced toxicity, whereby key cellular processes such as proliferation, migration, and apoptosis are restored. To demonstrate our concept, we employed CRISPR-dCas9 epigenome editing tools, utilizing catalytically deactivated Cas9 fused with either DNA methyltransferases or ten-eleven translocation ( Show less