This research verified the in vitro study of short chain fatty acids (SCFAs) extract and cell-free supernatant (CFS) produced from various probiotic Lactobacillus strains and evaluated its function of Show more
This research verified the in vitro study of short chain fatty acids (SCFAs) extract and cell-free supernatant (CFS) produced from various probiotic Lactobacillus strains and evaluated its function of anti-obesity. The production of SCFAs produced from the combination of L. paracasei SD1 and L. rhamnosus SD11 provided the highest SCFAs content at fermentation at 24 h and 45°C. The CFS exhibits a markedly stronger ability to prevent adipogenesis compared to the extract. Specifically, the combination of L. paracasei SD1 and L. rhamnosus SD11 demonstrates the highest suppression of lipid accumulation in 3T3-L1 adipocytes. It was observed that the CFS had a dose-dependently inhibitory effect on adipocyte differentiation, which was linked to a significant downregulation of gene expression levels of C/EBP-β, C/EBP-α, PPARγ, FAS, and LPL. The findings revealed the possibility of utilizing the CFS as a functional food due to its anti-obesity abilities by suppression of adipogenesis/lipogenesis in 3T3-L1 adipocytes. Show less