👤 Kathleen Lacy

Atherosclerosis is a chronic immunometabolic disease driven by lipid accumulation and immune cell infiltration. Macrophages and T cells play key roles throughout plaque development. Galectin-1 (Gal-1), a glycan-binding protein, modulates immune functions in these cells and has been reported to attenuate atherosclerosis, though its mechanisms remain incompletely understood. Here, we investigated the effects of Gal-1 on macrophages and T cells during plaque formation. Effects of Gal-1 on atherosclerosis, macrophages and T cells during lesion formation were studied in Apoe Gal-1 treatment reduced lesion size and increased circulating IL-10 levels, inversely correlating with plaque burden. Unexpectedly, IL-10 neutralization also mitigated atherosclerosis, indicating that its action is at least partially IL-10-independent. In plaques, Gal-1 promoted anti-inflammatory macrophage phenotypes, mirrored by a quiescent metabolic and anti-inflammatory profile in foamy macrophages ex vivo. The use of the Gal-1 Gal-1 protects against atherosclerosis associated with reprogramming macrophages and tuning T cell immunity through glycan-dependent and -independent pathways. Show less

Prior research has utilised person-centred approaches to identify parent feeding profiles distinguished by controlling and structure-based practices, but less research has examined autonomy support-based practices, or how social and family contextual factors differ between feeding profiles. This study aimed to identify profiles of parents with similar patterns of feeding practices and to examine whether profiles differ on family contextual factors. In 2022, 989 UK parents of children aged 3-6 years (M = 4.1 years) completed an online survey, which included the Comprehensive Feeding Practices Questionnaire (CFPQ), measuring parental feeding practices, and validated questionnaires capturing family contextual variables. Latent Profile Analysis (LPA) was conducted to identify parent feeding profiles using the CFPQ. A MANCOVA assessed differences in family contextual variables between profiles. LPA identified three profiles based on common model fit indices and theoretical considerations. Profile 1 'moderate control' (25.2%) showed moderate use of controlling practices and low use of structure-based and autonomy support-based practices. Profile 2 'structured and supportive' (29.6%) showed low use of controlling practices and high use of structure-based and autonomy support-based practices. Profile 3 'using everything' (45.2%) showed high use of all three types of feeding practices. Parents in the 'moderate control' profile had significantly lower parental wellbeing and reported more barriers of time and energy for meal planning compared to other profiles. In contrast, parents in the 'structured and supportive' profile had significantly lower household chaos and lower parental stress. Mothers had a higher proportion of membership to the 'structured and supportive' profile (33.9%) compared to other profiles, whereas fathers had a higher membership proportion to the 'using everything' profile (60.9%). Future interventions should be tailored to parent feeding practice profiles and associated family contextual factors. Show less

How distinct mesodermal lineages - extraembryonic, lateral, intermediate, paraxial and axial - are specified from pluripotent epiblast during gastrulation is a longstanding open question. By investigating AXIN, a negative regulator of the WNT/β-catenin pathway, we have uncovered new roles for WNT signaling in the determination of mesodermal fates. We undertook complementary approaches to dissect the role of WNT signaling that augmented a detailed analysis of Show less