👤 Tayyaba Afsar

This study aimed to investigate the effects of GAA supplementation in diets differing in ME levels on productive performance, egg quality, blood parameters, yolk fatty acid profiles, hepatic expression of genes related to lipid metabolism, gut morphology, and nutrient digestibility in laying hens during their post-peak production phase. Over a 12-week period (52-64 weeks of age), 288 laying hens were randomly assigned to 6 treatments. Each treatment consisted of 8 replicates, with 6 hens per replicate. The experimental treatments were assigned in a 2 × 3 factorial arrangement, comprising 2 levels of dietary ME (a recommended level and a low level, the latter characterized by a 100 kcal/kg reduction in ME) and 3 levels of GAA supplementation (0, 0.6, and 1.2 g/kg). The results showed significant interaction effects (P < 0.05) between GAA supplementation and dietary ME levels on laying rate, egg mass, feed conversion ratio, crude protein digestibility, and AMEn. In hens fed the low-ME diet, GAA supplementation, particularly at 1.2 g/kg, significantly improved laying performance. Moreover, at both 0.6 and 1.2 g/kg under low-ME conditions, GAA significantly enhanced crude protein digestibility and AMEn. The low-ME diet was associated with decreased expression of key lipogenic genes, including sterol regulatory element-binding transcription factor 1 (SREBF1), acetyl-coenzyme A carboxylase (ACC), and fatty acid synthase (FAS), alongside increased expression of genes involved in fatty acid oxidation, such as peroxisome proliferator-activated receptor alpha (PPARα) and carnitine palmitoyltransferase 1 (CPT1). Regardless of ME content, GAA supplementation linearly improved eggshell strength, enhanced the polyunsaturated-to-saturated fatty acid ratio in the yolk, elevated serum levels of creatine and total antioxidant capacity, improved intestinal morphology, and increased radical scavenging activity in the yolk (P < 0.05). Furthermore, GAA supplementation linearly increased the relative mRNA expression of several metabolic genes, including SREBF1, ACC, PPARα, and ApoB (P < 0.05). In conclusion, GAA supplementation enhanced productive performance in low-ME diets and exerted positive effects on egg characteristics and lipid metabolism, regardless of dietary ME content. Show less

Colon cancer is reported as third most prevalent malignancy worldwide, while sericin being an antioxidant, is now used in biomedical applications due to its biochemical characteristics and has shown potential efficacy to treat colon cancer. Sericin was isolated by the degumming process followed by the characterization by using FTIR, UV, XRD, and SEM techniques to confirm the successful synthesis of SAgNPs and SChiAgNPs. The male Balb C mice were then divided into 13 groups. Group 1: Control, Group 2: DMH (20 mg/kg) (injected (IP) thrice a week for 14 weeks). Groups 3,4,5: Sericin (S) (100 mg/kg), Sericin silver nanoparticles (SAgNPs) (100 mg/kg), and Sericin Chitosan silver nanoparticles (SChiAgNPs) (100 mg/kg) were given orally for 14 weeks respectively. Groups 6,7,8,9 were considered as preventive groups and were given DMH (IP) + 5-FU (IP), DMH(IP) + S (orally), DMH (IP) + SAgNPs (orally), DMH (IP) + SChiAgNPs (orally) respectively for the period of 14 weeks Groups 10,11,12,13 were considered as treatment groups and were given 5-FU (5 mg/kg) (IP), (S) (100 mg/kg) (orally), (SAgNPs) (100 mg/kg) (orally), (SChiAgNPs) (100 mg/kg) (orally) for a period of first 7 weeks after 7 weeks of DMH administration (IP). After 14 weeks period study, blood samples and colon tissue were used for the analysis of biochemical markers i.e., CEA, CA19-9, TIMP-1, and IL-6, IL-8, IL-27, SOD, CAT, GR, GSH, GST, MDA and MMP-7 via ELISA and histopathological analysis. The UV absorption peaks obtained at 435 and 463 nm indicated the formation of SAgNPs and SChiAgNPs formation respectively. FTIR spectra peaks obtained, indicate NH stretching of primary and secondary amine group), (NH stretching of amine salt) (N=C=S stretching of thiocyanate compound), (CC stretching of alkene), (NO stretching of nitro compound), (SO stretching of sulfonyl chloride), (CN stretching of amine) and (C-O-O stretching) for sericin, SAgNPs, and SChiAgNPs, confirming, the presence of theses functional groups. The XRD pattern revealed that SAgNPs and SChiAgNPs had structure crystalline structures. EDX characterization peaks for SAgNPs indicated the presence of silver along with other elements including; calcium, oxygen carbon, while EDX characterization peaks for SChiAgNPs indicated the presence of silver along with other elements including; oxygen, carbon, sodium, phosphorus, Sulphur and chlorine. SEM analysis showed that SAgNPs are of spherical shape, while the SChiAgNPs displayed the rectangular shape. The results for biomarker analysis indicated significantly elevated levels of CEA, CA19-9, TIMP-1, IL-6, IL-8, IL-27, MDA, and MMP-7 in DMH treated group (p ≤ 0.001) which were decreased significantly in SChiAg(T) (p ≤ 0.001). In contrast, levels of SOD, GR, GSH, CAT and GST were reduced significantly in DMH treated group, which were increased significantly in SChiAg(T) (p ≤ 0.001). The histopathological analysis of proximal and distal parts of colon tissue of the DMH-treated group showed low-grade dysplasia (LGD), and high-grade dysplasia (HGD) while SChiAgNPs improved the histopathological changes induced by DMH. The findings suggest that Sericin Chitosan conjugated silver nanoparticles showed their efficacy against DMH-induced colon cancer, making a potential future in the anticancer research field. Show less