👤 Amr A K Mousa

Ketosis is one of the most important metabolic disorders occurring in dairy cows after calving, which is caused by a negative energy balance during the peripartum period or around parturition. The current study focused on the diagnostic significance of the clinical scoring system, with serum lipid profiles, mainly lecithin cholesterol acyltransferase (LCAT) and apolipoprotein B-100 (ApoB-100), as recovery indicators in ketotic dairy cattle using the hormonal-steroids-carbohydrate therapeutic protocol. The study also discussed the correlation coefficient between serum apo-B100 and serum non-esterified fatty acids (NEFAs) in pretreated and post-treated ketotic cattle. In this study, 50 ketotic cattle from various dairy farms. The ketotic cows were subjected to a hormonal-steroids-carbohydrate therapeutic regimen extended for a continuous 5 days, including mainly insulin, dexamethasone, and carbohydrate replacement therapy (Glucose solution 50% alternative with dextrose solution 50%) with drenching of propylene glycol. and vitamin B The ketotic dairy cattle needed a maximum 30-day follow-up period post-therapy to restore their physiological status for clinical scoring data (7 days needed) and most serum lipid indices [14 days needed, i.e., apoB-100, β-hydroxy butyric acid, NEFA, glucose, cholesterol, and AST]. A 30-day post-therapy as a follow-up period was urgently required to restore their reference values of serum LCAT. The results of this study indicated that serum LCAT activity and ApoB-100 were the most sensitive indicators of the efficacy of therapy and the restoration of the physiological status. The study revealed a significant negative correlation between serum NEFA and apoB-100 in ketotic cattle. The results of this study indicated that serum LCAT activity and ApoB-100 were the most sensitive indicators of the efficacy of therapy and the restoration of the physiological status. This could be seen by an obvious improvement in serum lipid profiles, mainly LCAT and apoB-100, and clinical findings as well as clinical coring data. The study identified a significant negative correlation between NEFA and apoB-100 in ketotic cattle. Exposure of diseased ketotic dairy cattle to this specific therapeutic strategy had a great impact on their recovery from ketosis and improved their health and productivity status. The current study concluded the diagnostic significance of the clinical scoring system (Appetite score, rumen filling score, manure digestion score, and manure condition score), and serum lipid profiles, mainly LCAT and apoB-100, as recovery indicators in ketotic dairy cattle using hormonal-steroids-carbohydrates therapeutic protocol. Show less

HBsAg incidence in Egypt is at a range of 2 to 7%. Antiviral immunity is linked to interleukin-27 (IL27), a cytokine that is produced by two genes: EBI3 and p28. IL-27 gene SNPs can alter the susceptibility to infection of HVB by impacting the production and/or function of cytokines. The study aimed to examine the impact of the IL-27 SNPs on the progression of HBV infection among Egyptian individuals. This study included a total 112 patients infected with HBV, and 50 healthy individuals served as controls. The link between the IL-27 SNPs (rs181206 T/C and rs17855750 T/G) and HBV was investigated using real-time PCR. There was no significant correlation between fibrosis stages and the distribution of IL-27 rs181206 T/C and rs17855750 T/G genotypes among HBV patients. Results indicated minimal disparity in the distribution of haplotypes among the study groups. No significant difference in the frequency of the CG, CT, TT, TG, and haplotypes between the groups. This study found no correlation between the presence of IL-27 rs1812006 and IL-27 rs17855750 SNPs and the HBV chronicity. Show less