Cholesterol deficiency (CD) in Holstein cattle, caused by a loss-of-function mutation in the A total of 188 cows were classified by lactation: 1 The heterozygous CD genotype was identified in 17.02% o Show more
Cholesterol deficiency (CD) in Holstein cattle, caused by a loss-of-function mutation in the A total of 188 cows were classified by lactation: 1 The heterozygous CD genotype was identified in 17.02% of the population, with wild-type and mutant allele frequencies of 0.91 and 0.09, respectively. Non-carriers showed marginally higher fat, protein, and cholesterol levels, with a statistically significant difference in fat content (p = 0.04). When stratified by lactation, significant differences were observed for fat content in the 1 This study confirms the presence of the CD-associated Show less
Genotype analysis was done for 45 samples. The research results were obtained with the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The 1,200-bp PCR product wa Show more
Genotype analysis was done for 45 samples. The research results were obtained with the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The 1,200-bp PCR product was cut with Upon analyzing the data, it was found that 37.8% ( After analysis of the study results, a statistically significant difference was observed between cats with DM and clinically healthy cats in the comparison of their BCS ( Show less