Cholesterol deficiency (CD) in Holstein cattle, caused by a loss-of-function mutation in the A total of 188 cows were classified by lactation: 1 The heterozygous CD genotype was identified in 17.02% o Show more
Cholesterol deficiency (CD) in Holstein cattle, caused by a loss-of-function mutation in the A total of 188 cows were classified by lactation: 1 The heterozygous CD genotype was identified in 17.02% of the population, with wild-type and mutant allele frequencies of 0.91 and 0.09, respectively. Non-carriers showed marginally higher fat, protein, and cholesterol levels, with a statistically significant difference in fat content (p = 0.04). When stratified by lactation, significant differences were observed for fat content in the 1 This study confirms the presence of the CD-associated Show less
Genotype analysis was done for 45 samples. The research results were obtained with the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The 1,200-bp PCR product wa Show more
Genotype analysis was done for 45 samples. The research results were obtained with the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The 1,200-bp PCR product was cut with Upon analyzing the data, it was found that 37.8% ( After analysis of the study results, a statistically significant difference was observed between cats with DM and clinically healthy cats in the comparison of their BCS ( Show less
Variation in carotenoid bioavailability at individual and population levels might depend on host-related factors where genetic variation has a part to play. It manifests itself through the proteins in Show more
Variation in carotenoid bioavailability at individual and population levels might depend on host-related factors where genetic variation has a part to play. It manifests itself through the proteins involved in carotenoid intestinal absorption and metabolism, blood lipoprotein transport, or tissue uptake. This study aims to identify novel SNPs which could be associated with carotenoid serum concentrations. A total of 265 self-reported healthy individuals of Lithuanian origin were genotyped (Illumina HumanOmniExpress-12v1.0 or v1.1 and Infinium OmniExpress-24v1.2 arrays) and fasting blood serum concentrations of β- and α-carotene, β-cryptoxanthin, lycopene, lutein, and zeaxanthin were measured (Shimadzu Prominence HPLC system). According to the individual carotenoid concentrations, the cohort was subdivided into quartiles. Q1 and Q4 were used for the following association analysis. The set of 2883 SNPs in 109 potential candidate genes (assumed for a direct or indirect role in carotenoid bioavailability) was analyzed. Liver X receptor alpha ( Show less